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Objective: To identify the differential metabolic small molecule substances in Cervi Cornu Pantotrichum (CCP) of different segments based on UPLC-QTOF/MS metabonomics technology, and analyze the metabolic pathways involved by differential metabolites combined with histological observation. Methods: The standard trident antler of sika deer (Cervus nippon) were treated by freeze-drying. The tissue morphology was observed by section. The tissue morphology was divided into three sections from top to bottom, VAU, VAM, and VAB. The differential metabolites were analyzed by UPLC-QTOF/MS technology, principal component analysis, and least squares discriminant analysis. Results: A total of 124 kinds of metabolic small molecular substances were identified in CCP. Based on the FC value of differential metabolites higher than 2.2, 16 substances with different relative contents in different parts of CCP were further screened, and the relative content of each substance in different parts were compared. Conclusion: This experiment is based on non-targeted metabonomics to analyze the composition of metabolic small molecules in freeze-dried CCP. By comparing the adjacent zones, it is found that there are some differences in endogenous metabolites among the zones, which provides data support for revealing their functions.
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Objective To compare the contents of mineral elements (Na, Mg, Al, P, K, Ca, Mn, Fe, Co, Ni, Cu, Zn, Se, Cr, As, Cd, Ag, Pb, Ba, and Au) in different parts of Cervi Cornu Pantotrichum (CCP) with different processing methods, with aims to provide a guidance for the effective utilization and determination of medicinal value of CCP. Methods The three-branched velvet antler collected from Jilin Province was pretreated by microwave digestion and wet digestion, and the content of elements was determined by inductively coupled plasma mass spectrometry (ICP-MS). Results The results of detection of Cd and Ag in samples were all less than 0.001 μg/kg. Higher contents of Na, Mg, Al, P, K, and Ca were observed in antler samples especially the content of Ca which increased sharply from wax to bone section. The content of Ca reached to 14.86%-16.44% in the bone section. The contents of Na, Mg, Al, P, K and Ca were significantly different in different sections (P < 0.05). The contents of Mn, Cu, Zn, and Ba were relatively higher than Fe, Co, Ni, Se, and Cr in antlers. The contents of Cr, As, Au, and Pb were very low within the limits of heavy metals of food. Se was rich in antler sample which contains 260.35-357.29 μg/kg in wax section. Drying and freeze-drying treatments had significant differences in Al and other elements (P < 0.05). The overall level presented that the contents of Al, P, and Ca in the boiled fry were higher than those in frozen dried antler, however, Na, Mg, and K were the opposite. Conclusion Different initial processing methods affected the contents of some mineral elements in three-branched velvet antler, and different sections showed different rules. The content of mineral elements in different sections of velvet antler varied greatly. This study provides a theoretical basis for the fine use of antler in different sections.
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AIM:To evaluate the effect of exogenous hydrogen sulfide(H2S)on the expression of NLRP3 in-flammasome in hepatocytes.METHODS:The hepatocytes L 02 and SMMC-7721 were used to establish the model of inflam-mation by stimulating with lipopolysaccharide(LPS)at different concentrations in vitro.The expression of NLRP3 inflam-masome in the hepatocytes was detected by Western blot and the cell viability was measured by MTT assay for determining appropriate concentration of LPS.The hepatocytes were divided into 4 groups:the cells in control group were incubated with normal medium for 18.5 h;the cells in LPS group were incubated with normal medium for 0.5 h followed by 100 μg/L LPS for 18 h;the cells in LPS+H2 S group and H 2 S group were incubated with 200μmol/L sodium hydrosulfide hydrate(NaHS)for 0.5 h followed by 100 μg/L LPS or normal medium for 18 h,respectively.The protein expression of NLRP3 and caspase-1 in the cells of every group was determined by Western blot.RESULTS:Compared with control group ,the protein expression of NLRP3 and caspase-1 increased significantly in LPS group(P<0.05)and had no significant change in H2S group.Compared with LPS group,the protein expression of NLRP3 and caspase-1 in LPS+H2S group decreased significantly(P<0.05).CONCLUSION:In hepatocytes,exogenous H2S suppresses the expression of NLRP3 inflamma-some.